The
Preparation of an Artificial Red Blood Cell Substitute by
W/O/W Double Emulsion Methods
QIU Wei,
MENG Fan-Tao, MA Guang-Hui, SU Zhi-Guo*
( State Key Laboratory of Biochemical Engineering, Institute
of Process Engineering, the Chinese
Academy of Sciences, Beijing 100080, China )
Abstract A method of preparing methoxypoly
(ethylene glycol)-poly-DL-lactide (PELA) microcapsule containing bovine
hemoglobin (BHb) by using W/O/W double emulsion-solvent diffusion process for
use as artificial red cells was developed. The results suggested that
preparation conditions such as solidification method, stirring rate, type of
organic solvent had significant influence on the encapsulation efficiency and
the activity of bovine hemoglobin. When ethyl acetate was used as the organic
solvent and double emulsion-solvent diffusion process was used, there was no
significant influence on the activity of hemoglobin if stirring rate was lower
than 9000 r/min. High stirring rate, 12 000 r/min, decreased the P
Key words artificial red cells; methoxypoly(ethylene glycol)-poly-DL-lactide; bovine hemoglobin; microcapsule
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